integrinβ1  (Galectin Therapeutics)

Journal: Discover Oncology

Article Title: Effect of fibrinogen like protein 1 on cisplatin-resistance in laryngeal carcinoma through targeting integrinβ1 in vivo and in vitro

doi: 10.1007/s12672-025-03337-x

Figure Lengend Snippet: Integrinβ1 affected CDDP resistance and was regulated by FGL1 in TU212-R cells. A The expression of FGL1 and Integrinβ1 in NP69, TU212 and TU212-R cells was detected by western blot assay. B The cell viability of TU212-R cells was assessed after FGL1 knockdown and treatmentwith Pyrintegrin. C , D Expression levels of FGL1 and Integrinβ1 in TU212-R cellsafter FGL1 knockdown and treatmentwith Pyrintegrin. Grey values were analyzed by Image J software, with expression normalized to GAPDH, n=3.ns indicates no significant difference, ** P < 0.01, *** P < 0.001.

Article Snippet: The interaction between Integrinβ1 and Galectin-1 can promote drug resistance of breast cancer cells [ ].

Techniques: Expressing, Western Blot, Knockdown, Software

Journal: Discover Oncology

Article Title: Effect of fibrinogen like protein 1 on cisplatin-resistance in laryngeal carcinoma through targeting integrinβ1 in vivo and in vitro

doi: 10.1007/s12672-025-03337-x

Figure Lengend Snippet: Downregulation of FGL1 inhibited the migration and invasion abilities of TU212-R cells through regulating Integrinβ1. The migration and invasion abilities of TU212-R cells were determined by wound healing assay (scale bar: 50 μm) (A) and transwell assay (scale bar: 50 μm) (B) after FGL1 knockdown and treatment with Pyrintegrin. n=3, ns means no significant difference, ** P < 0.01, *** P < 0.001.

Article Snippet: The interaction between Integrinβ1 and Galectin-1 can promote drug resistance of breast cancer cells [ ].

Techniques: Migration, Wound Healing Assay, Transwell Assay, Knockdown

Journal: Discover Oncology

Article Title: Effect of fibrinogen like protein 1 on cisplatin-resistance in laryngeal carcinoma through targeting integrinβ1 in vivo and in vitro

doi: 10.1007/s12672-025-03337-x

Figure Lengend Snippet: Tumorigenicity of TU212-R in vivo. A Representative images of mice bearing TU212-R tumors from different treatment groups. The tumor volume growth curves ( B ) over the study period and the tumor weight ( C ) after injections of different treatment groups. D Immunohistochemical staining for Ki67, FGL1, Integrinβ1 and P-gp proteins in different xenograft tumor tissues (scale bar: 100 μm). The positive area was quantified by Image J software. n = 5, ns means no significant difference, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The interaction between Integrinβ1 and Galectin-1 can promote drug resistance of breast cancer cells [ ].

Techniques: In Vivo, Immunohistochemical staining, Staining, Software

Journal: Discover Oncology

Article Title: Effect of fibrinogen like protein 1 on cisplatin-resistance in laryngeal carcinoma through targeting integrinβ1 in vivo and in vitro

doi: 10.1007/s12672-025-03337-x

Figure Lengend Snippet: The expression of Integrinβ1 and resistance proteins regulated by FGL1 affected apoptosis in vivo. (A) Representative images of TUNEL staining in different xenograft tumor tissues (scale bar: 200 μm). (B) The apoptosis incidence was measured by Image J software. (C) Expression levels of FGL1, Integrinβ1, MRP1 and P-gp in different xenograft tumor tissues were evaluated by western blot assay. n=5, ns indicates no significant difference, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The interaction between Integrinβ1 and Galectin-1 can promote drug resistance of breast cancer cells [ ].

Techniques: Expressing, In Vivo, TUNEL Assay, Staining, Software, Western Blot

Journal: Micromachines

Article Title: A Microfluidic-Based Cell-Stretching Culture Device That Allows for Easy Preparation of Slides for Observation with High-Magnification Objective Lenses

doi: 10.3390/mi16010093

Figure Lengend Snippet: Effects of cyclic stretching on integrinβ1 and vinculin localization in OP9 cells. Fluorescence images of integrinβ1 (green), f-actin (red), and vinculin (gray) in OP9 cells cultured on the device after 72 h under static conditions followed by 24 h under ( A ) static or ( B ) cyclic stretching conditions (0.5 Hz) photographed with a 100× oil immersion objective lens. Confocal images were captured every 0.48 µm along the z-axis to create z-stacks (14 slices) for maximum-intensity projection (scanning zoom 2×). The right figure shows the fluorescence intensity–distance profiles of integrinβ1 (green), f-actin (red), vinculin (gray), and nuclei (blue) on lines ( a – d ) drawn on the microscope image (MIP).

Article Snippet: For integrinβ1, vinculin, f-actin, and nuclear staining, 0.5% BSA was added with anti-integrinβ1 antibody (0.4 μg/mL; SC-19656, Santa Cruz Biotechnology, Dallas, TX, USA), anti-vinculin antibody (4 μg/mL; 26520-1-AP, Proteintech, Rosemont, IL, USA), and rhodamine phalloidin (1.65 μM; R4159, Thermo Fisher Scientific) and incubated overnight at 4 °C.

Techniques: Fluorescence, Cell Culture, Microscopy

Journal: iScience

Article Title: Exosomal Galectin-3 promotes peritoneal metastases in gastric adenocarcinoma via microenvironment alterations

doi: 10.1016/j.isci.2024.111564

Figure Lengend Snippet:

Article Snippet: Integrinβ1 , Santa Cruz , Cat# sc-8978, RRID: AB_2130101.

Techniques: Microarray, Recombinant, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Staining, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Software